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Spoilers ahead, obviously.

1. Ezio becomes an assassin (AC2)

This is the moment Ezio pulls on the famous hood and goes from mischievous street brawler to vengeful assassin. Jesper Kyd's beautiful score makes this scene extra special.

2. 16's sacrifice (AC: Revelations)

You're never quite sure of subject 16's intentions until the Animus begins deleting 'excess data', including Desmond, and he sacrifices himself to save his successor. Poignant.

3. Ezio 'meets' Altair (AC: Revelations)

Ezio and his ancestor, Altair, finally meet each other in the flesh. Well, sort of. It's an emotional moment, especially if you've played the first game, and a great end to Revelations.

4. Ezio the minstrel (AC: Revelations)

The annoying minstrels became notorious in Assassin's Creed 2, so in Revelations Ubisoft turned the tables and made you become one yourself. A rare light-hearted moment.

5. 16's secret revealed (AC)

With his newfound eagle vision powers, Desmond goes to his room and sees a series of mysterious notes and glyphs left by his predecessor, the enigmatic subject 16.

6. Meeting uncle Mario (AC2)

This is a very silly moment, but we couldn't help but crack a smile. Ubisoft pay homage to Nintendo's Italian mascot when Ezio runs into one of his most trusted allies, uncle Mario.

7. The truth revealed (AC2)

To unlock all the pieces of this video left by subject 16, you have to solve 20 glyph puzzles. Its content is one of AC's biggest mysteries, and the subject of much debate among fans.

8. Wielding the apple (AC: Revelations)

The mysterious Apples of Eden is at the core of the AC mythology. It grants its bearer incredible powers, which you got to try out for yourself in Altair's fifth flashback sequence.

9. Lucy's end (AC: Brotherhood)

Under the influence of an Apple of Eden and the mysterious Juno, Desmond kills Lucy with his hidden blade and then falls into a coma. A shocking end to the game.

10. Return to Masyaf (AC: Revelations)

Ezio visits Masyaf, the once great headquarters of the Assassin order, and Altair's old stomping ground. His journey is captured in this absolutely stunning CG intro movie.

11. The solar flare (AC: Revelations)

In Revelations, we finally see the event that destroyed humanity and the First Civilization; a massive solar flare. Desmond's goal is now to stop a second one from destroying Earth.

12. Street fighter (AC2)

Before Ezio became the assassin we know and love, he was usually found fighting on the streets of Florence with local gangs. This was a great way to introduce the cocky womaniser.

13. Cesare falls (AC: Brotherhood)

Cesare Borgia was one of the most despicable villains in the Assassin's Creed series. In Brotherhood we got to toss him off a roof after killing him. Good riddance.

14. Assassinating the Pope (AC2)

It's okay; he's an evil, corrupt Pope. Assassin's Creed 2 is perhaps the only game in history that ends with a fist fight with a High Pontiff. Take that, your grace.

15. Old Altair (AC: Revelations)

Altair, now an old man, returns to Masyaf. He's slow and weak, and can't fight for himself. It was interesting to feel so powerless in an Assassin's game.

16. Monteriggioni destroyed (AC: Brotherhood)

We spent almost 10 hours fully upgrading Monteriggioni in Assassin's Creed 2, which made seeing it being invaded and ruined in Brotherhood hard to bear. Cesareeeee!

17. The flying machine (AC2)

Leonardo da Vinci's rickety flying machine gives Ezio the chance to soar freely across the city like a bird. It's a shame the flight's spoiled by all those archers.

18. Desmond's memories (AC: Revelations)

These puzzle sections were part Portal, part LittleBigPlanet. They filled in a lot of Desmond's previously unknown backstory, including his ties to the Assassins order.

19. Animus Island (AC: Revelations)

So that's what it looks like inside the Animus' operating system. It's represented as some bizarre desert island full of weird black monoliths, and the 'ghost' of subject 16.

20. Altair flashback (AC2)

In Assassin's Creed 2, Desmond briefly steps into the memories of Altair and we see a mysterious sequence where he climbs a tower and meets Maria, which is explained in Revelations.